%0 Journal Article
%T Children’s Inflammatory Bowel Disease and Methanogenic Archaea: Links to Disease Classification and Activity
%A Ahmed K. El Sherif
%A Dina M. Saleh
%A Omar A. Youssef
%J Bulletin of Pioneering Researches of Medical and Clinical Science
%@ 3006-2659
%D 2022
%V 2
%N 1
%R 10.51847/I0V1glGDBV
%P 134-144
%X Inflammatory bowel disease (IBD) involves disruptions in the gut microbial ecosystem, yet research on methanogenic archaea in children is scarce. This study investigated the presence and abundance of total methanogenic archaea and three specific groups—Methanobrevibacter smithii (Mb. smithii), Methanosphaera stadtmanae (Ms. stadtmanae), and the Methanomassiliicoccales order—in the fecal samples of pediatric patients with Crohn’s disease (CD) and ulcerative colitis (UC), considering both active and inactive disease states. Quantitative real-time PCR data were analyzed in relation to disease type and activity, assessed via the Pediatric Crohn’s Disease Activity Index (PCDAI), Pediatric Ulcerative Colitis Activity Index (PUCAI), and fecal calprotectin (FCP), and compared with healthy children. Total methanogen counts were significantly lower in both CD and UC patients than in controls, with UC showing the lowest prevalence. In inactive UC, Mb. smithii levels were reduced, and Ms. stadtmanae abundance displayed a moderate positive correlation with FCP concentrations. These findings suggest that methanogenic archaea profiles vary according to IBD type and activity in pediatric patients.
%U https://bprmcs.com/article/childrens-inflammatory-bowel-disease-and-methanogenic-archaea-links-to-disease-classification-and-m7nrvqpap4ymxpm