TY  - JOUR
T1  - Modulation of Alveolar Macrophage Inflammation in Sarcoidosis by Bone Marrow-Derived MSCs
A1  - Soojin Lee
A1  - Jihyun Oh
A1  - Eun-Sun Lim
JF  - Bulletin of Pioneering Researches of Medical and Clinical Science
JO  - Bull Pioneer Res Med Clin Sci
SN  - 3006-2659
Y1  - 2022
VL  - 2
IS  - 1
DO  - 10.51847/ohqghFQAGp
SP  - 85
EP  - 94
N2  - Sarcoidosis is a severe inflammatory disorder that can involve multiple organs, particularly the lungs and lymph nodes. Bone marrow-derived mesenchymal stromal cells (MSCs) have the ability to “reprogram” various macrophage populations toward an anti-inflammatory phenotype. This study investigated whether alveolar macrophages from sarcoidosis patients exhibit a similar anti-inflammatory response when co-cultured with MSCs. Bronchoscopy and bronchoalveolar lavage (BAL) were performed on fifteen sarcoidosis patients and eight healthy controls. Unselected BAL cells, comprising 70–94% macrophages, were isolated and cultured with or without MSCs derived from healthy donors. After stimulating the cultures with lipopolysaccharide, supernatants were collected to measure levels of interleukin-10 (IL-10) and tumor necrosis factor-alpha (TNF-α). In two additional sarcoidosis patients, flow cytometry was used to evaluate intracellular cytokines and surface markers to validate the findings. In nine out of eleven sarcoidosis samples, co-culture with MSCs resulted in decreased TNF-α (indicative of pro-inflammatory M1 activity) and increased IL-10 (indicative of anti-inflammatory M2 activity). BAL cells from control subjects displayed minimal changes in cytokine production. Flow cytometric analysis in the additional patients confirmed a shift of alveolar macrophages from a pro-inflammatory (M1) to an anti-inflammatory (M2) state following MSC co-culture. These findings indicate that alveolar macrophages, like other macrophage populations, can adopt an anti-inflammatory phenotype in response to MSC interaction. We propose that administering MSCs to the airways could potentially reduce lung inflammation and lower the need for corticosteroids in patients with sarcoidosis.
UR  - https://bprmcs.com/article/modulation-of-alveolar-macrophage-inflammation-in-sarcoidosis-by-bone-marrow-derived-mscs-olyrvgviy4otnkk
ER  -