The progression of periodontal disease is influenced by numerous variables, with dysbiotic microbial flora being the most crucial, exhibiting varying levels of pathogenic potential. Swift bacterial colonization within the subgingival niche can substantially alter the clinical presentation of the periodontium. This systematic review highlights the novel application of loop-mediated isothermal amplification (LAMP) for fast, multiplex identification of microorganisms associated with periodontal conditions. The main advantage of LAMP compared to conventional nucleic acid detection techniques such as polymerase chain reaction (PCR or qPCR) lies in its capacity to simultaneously identify multiple pathogens with superior sensitivity. In contrast with standard culture-based microbiological methods, LAMP reduces the diagnostic period from several days to only minutes, facilitating rapid species identification and quantification of microbial populations. Because this approach demands minimal laboratory infrastructure, it can easily be applied in outpatient practice. The method allows near-instant evaluation of periodontal health and enables assessment of potential complications during therapy (e.g., uncontrolled inflammatory spread), which holds significant clinical relevance.
